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Mouse Mucin 2 (MUC2) ELISA Kit

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Product Name:

Mouse Mucin 2 (MUC2) ELISA Kit

Cat.#:

JL47609

Regulatory Status:
Alternative:

MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.31ng/mL
Specificity:
This kit recognizes Mouse MUC2 in samples. No significant cross-reactivity or interference between Mouse MUC2 and analogues was observed.
Sample Type:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Cancer
  • Mouse Mucin 2 (MUC2) ELISA Kit
  • Mouse Mucin 2 (MUC2) ELISA Kit
  • Mouse Mucin 2 (MUC2) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| MUC2

Mucin 2, oligomeric mucus gel-forming, also known as MUC2, is a protein that is encoded by the MUC2 gene.This gene encodes a member of the mucin protein family. The protein encoded by this gene, also called mucin 2, is secreted onto mucosal surfaces.It is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lining into the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins, polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins. This gel provides an insoluble mucous barrier that serves to protect the intestinal epithelium.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 50 25 12.5 6.25 3.12 1.56 0.78 0
OD 2.26 1.49 1.03 0.7 0.46 0.32 0.23 0.08
Corrected OD 2.18 1.41 0.95 0.62 0.38 0.24 0.15 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Mouse MUC2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Tissue Homogenates(n=8) 84-101 96
Cell Lysates(n=8) 92-105 102
Cell Culture Supernates(n=8) 95-108 105





 

| Linearity

Samples were spiked with high concentrations of Mouse MUC2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Tissue Homogenates(n=4) Cell Lysates(n=4) Cell Culture Supernates(n=4)
1 : 2 Range (%) 84-95 88-96 90-110
Average (%) 91 93 96
1 : 4 Range (%) 89-103 87-107 105-115
Average (%) 94 98 109







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
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